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Title : New Book Chapter: Investigation of Platelet Aggregation in Atherosclerosis
link : New Book Chapter: Investigation of Platelet Aggregation in Atherosclerosis

New Book Chapter: Investigation of Platelet Aggregation in Atherosclerosis

 

A new book on Atherosclerosis has just been published where we have contributed a chapter.

The title of the book is

Atherosclerosis: Methods and Protocols

edited by Dipak Ramji and

our chapter is

Investigation of Platelet Aggregation in Atherosclerosis

Platelet activation and aggregation is implicated in all stages of inflammation-related atherosclerosis from the initial steps of endothelial dysfunction and plaque formation, to plaque rupture and atherothrombotic events, such as acute coronary syndrome, myocardial infarction, and ischemic incidences. Platelet aggregometry assays are the mainstream for evaluating and monitoring platelet reactivity in such conditions and for the investigation of prophylactic and therapeutic approaches. The most established methodology is light transmittance aggregometry (LTA). Here we describe the appropriate preparation of platelet suspensions from human blood and the methodology of LTA-based assays that is used for basic and clinical research for monitoring and evaluating the activities of several thrombotic mediators, as well as determining the dose efficacy and safety of several pharmaceutical and nutraceutical compounds intended for therapeutic and prophylactic interventions for atherosclerosis.

Key words

Atherosclerosis Inflammation Thrombosis Platelet aggregation Light transmission aggregometry Platelet-activating factor Thrombin ADP Collagen Antiplatelet 

This chapter is dedicated to Prof. Constantinos A. Demopoulos, Εmeritus Professor at the University of Athens, Greece, our valued teacher, colleague, and friend.

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